Journal: Nature Communications

Article Title: A nutrient-responsive hormonal circuit mediates an inter-tissue program regulating metabolic homeostasis in adult Drosophila

doi: 10.1038/s41467-021-25445-2

Figure Lengend Snippet: a Co-immunolabeling of the CNS and the intestine from CapaR > mCD8::GFP flies with a custom generated CapaR antibody consistently showed an overlap between GFP (green) and CapaR (red) immunoreactivity in the mushroom body (MB) and neuronal somata of the CNS, as well as in the visceral muscles (VM) and enteroendocrine cells (EEs) restricted to distinct portions of the gut. Image was acquired using “Tile scan” in the ZEN software (Zeiss, Oberkochen, DE) with boundaries between tiles marked by dashed line. EC, enterocytes; CCR, copper-cell region. Scale bars = 200 µm. b – c Application of fluorophore-labeled Capa-1 (Capa-1-F, red) on the intestine shows specific and displaceable binding to CapaR > GFP (green) positive enteroendocrine cells (scale bar = 20 µm) and visceral muscles (scale bar = 40 µm). d Principle of in vivo aequorin luminescence-based functional calcium assay in Drosophila tissues. e – h Real-time changes in intracellular Ca 2+ concentration of tissues acutely dissected from flies expressing a UAS-apoaequorin transgene ( CapaR > aeq::GFP ). Cytosolic [Ca 2+ ] i levels (nM) in e proventriculus, f midgut, g hindgut and h brain upon Capa-1 stimulation (10 −7 M).

Article Snippet: To analyze long-term locomotor activity of individual flies, we used the Drosophila activity monitor system (TriKinetics) in combination with the pySolo software package and a custom MATLAB script (MATLAB R2016b, The MathWorks Inc, Natick, Massachusetts) as previously described .

Techniques: Immunolabeling, Generated, Muscles, Software, Labeling, Binding Assay, In Vivo, Functional Assay, Calcium Assay, Concentration Assay, Expressing